Fig. 2

The suppressive function of circMAPK1 in vitro. a CCK-8 assay was performed to evaluate proliferation ability of cells after upregulating or downregulating circMAPK1 in GC cells. b Colony formation assay was performed to evaluate proliferation ability of cells after upregulating or downregulating circMAPK1 in GC cells. c EdU assay was performed to evaluate proliferation ability of cells after upregulating or downregulating circMAPK1 in GC cells. Scale bar: 100 μm. d The effect of circMAPK1 on modulating cell cycle progression was evaluated by flow cytometry assay. e The effect of circMAPK1 on cell migration was examined by Transwell assay. Scale bar: 100 μm. Graph represents mean ± SD; *p < 0.05, **p < 0.01, and ***p < 0.001